Wasay Mohiuddin, Ph.D.
1. Embryonic Stem Cells Differentiation Towards Cardiac Lineage
In 1981, Martin Evans and Matthew Kaufman first derived the embryonic stem cells (ES cells) from mouse embryos. Later on in 1998 James Thomson derived the human ES cells. ES cells are pluripotent stem cells derived from the inner cell mass of a blastocyst, an early-stage embryo. The ES cells have the ability to differentiate into all three germ layers. The human ES cells raises some ethical issue which were solved in 2006 by Yamanaka by reprogramming the somatic cells into induced pluripotent stem cells (iPSC). In our lab we are mainly targeting ES cells and iPSC differentiation towards cardiac lineage using the famous hanging drop method. A number of small molecules and pathways were evaluated to study their role during cardiac differentiation.
Sox 2+ ES cells
Graduate Student: Bc. Štěpánka Skalová
Thesis: Differentiation of pluripotent stem cells into cardiomyocytes
The work fits into the long-term program directed to the study of tissue regeneration and the role of stem cells in this process. The study is based on societal needs of searching for alternative sources of cells suitable for cell therapy of myocardium diseases. The aim is to select the appropriate markers to identify fully mature cardiomyocytes and developmental stages of cells maturing into cardiac muscle cell lineage, and testing procedures for directed development of pluripotent stem cells toward the cardiac muscle cells in vitro.
2. Bone Marrow Stromal Stem Cells
Bone Marrow Stromal Stem Cells (BMSSC) are derived from the bone marrow. These are mesenchymal Stem cells possessing ability to differentiate into mesenchymal lineages and other germ layers lineages. The cells like other mesenchymal stem cells posses fibroblast like morphology. In our lab we are differentiating these cells towards cardiac lineages using small molecules. Another role of these cells is the use of their paracrine signaling in regenerative medicine particularly in cardiac cells regeneration. Which we have planned to with cardiosphere cells regeneration.
Hierlihy et al in 2002 reported that adult mouse myocardium retains an endogenous stem cell-like population that is activated during growth challenge. In 2003, Oh et al. isolated stem cell antigen-1-positive (Sca-1+) cells from the adult mouse myocardium. Beltrami et al. 2003 reported the presence of cardiac resident stem cells by isolating c-kit+ cells from adult rat hearts. These cardiac c-kit+ cells are described as self-renewing clonogenic multipotent cells that give rise to myocytes smooth muscle cells, and endothelial cells. Messina et al. 2004 were the first to isolate similar clonogenic cardiac stem cells from human biopsy tissues which differentiated into beating cardiomyocytes and osteocyte or adipocyte characteristics indicating their multipotency. Upon culture of cardiac tissue a layer of fibroblast-like cells is generated from the adherent explants, over which small phase-bright cells migrated and clustered together forming spheroids or cardiospheres (CSs). In our lab we are using these cardiosphere for regenerative medicine using stromal stem cells paracrine signaling.
Daniel Díaz Garcia, Ph.D.
1. Decellularized Scaffolds
Decellularization procedure allows to obtain a biostroma while retaining organ microstructure and original composition of structural and bioactive molecules that support cell attachment and organisation. Recently several groups established a proof of concept for utilization of decellularized bioscaffold reseeded with cells in reengineering of complex bioartificial organs like the heart (Ott et al., 2008), lung (Petersen et al., 2010) or liver (Uygun et al., 2010). Such organs are transplantable and functional as demonstrated by Uygun et al. 2010 in liver grafts or by Olausson et al. 2012 in veins. Our group is focused on muscle reconstruction using decellularized muscle scaffolds seeded with myogenic cells.
Ph.D. Student: Mgr. Hana Hrebíková
Thesis: Chemical decellularization
Graduate Students: Richard Adamčík, Pavel Beznoska, Kristýna Krejzlová
2. Primary Cilia
Primary cilia act as non-motile sensory strucutres that transduce signals from extracellular stimuli to a cellular response that regulates proliferation, differentiation, transcription, migration, polarity and tissue morphology. We study primary cilia on stem cells, myogenic cells and tumour cells.
Ph.D. Student: Mgr. Alžběta Filipová
3. Molecular Biology Methods
Primary Using moderns methods to modify stem cells behaviour and to study distribution of molecules in cells and tissues.
Graduate Student: Petra Hajzlerová
Bachelor Thesis: Immunohistochemistry as a tool for cell phenotypization.
Kapil Dev, Ph.D.
1. Cell Reprogramming
Cell reprogramming can be induced artificially through the introduction of exogenous factors, usually transcription factors. In this context, it often refers to the creation of induced pluripotent stem (iPS) cells from mature cells such as adult fibroblasts. This allows the production of stem cells for biomedical research, such as research into stem cell therapies, without the use of embryos. It is carried out by the transfection of stem-cell associated genes into mature cells using viral vectors such as retroviruses. We are working with mRNA reprogramming that does not disrupt DNA integrity.
Ph.D. Student: Rishikaysh Pisal
2. Directed Cell Differentiation
Stem cells including pluripotent iPS cells have a broad differentiation potential and such cells can sponaneously mature into multiple cell types. Our aim is to direct cell differentiation with the use chemokines, growth factors, small molecules or cell reprogramming toward the required phenotypes, namely neural cells.
Graduate Student: Bc. Tereza Švadláková
Thesis: Differentiation of pluripotent stem cells into neurons
Tomas Soukup, M.D., Ph.D.
Technology transfer manager
Ph.D. in Anatomy, histology and embryology
MSCs: Isolation, Characterization and Potential Clinical Applications
Organisational skills and competences
Team leader – PurStem project, see www.purstem.eu
Ph.D. student: MDDr. Tereza Suchánková Kleplová
Undergraduate student: Tereza Krchovová
Technical skills and competences
Experimental methods in histology, stem cell biology and embryology. Cell analysis incl. flow cytometry and cell separation techniques. qPCR – TATAA certified researcher. Technology scouting and technology transfer.
Computer skills and competences
MAC OS and OS Windows as well as Microsoft Office tools; advanced user. Standard skill in graphic editors. Image analysis (ImageJ, ImagePro Plus) and statistical software. Web design. Mendeley Desktop SW.