Equipment












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            1.The high-performance Oxygraph-2k respirometer (OROBOROS) allows the measurement of mitoc

              function (biotransformation energy pathways, respiratory complex activities) by measurin
              consumption using appropriate specific substrates, expanders and inhibitors. Mitochondri
              can be measured in isolated mitochondria, tissue homogenates, intact and permeabilized c

            2.The Agilent Seahorse XFe96 is a dedicated instrument that simultaneously measures key pa
              mitochondrial respiration (oxygen consumption rates = OCR) and glycolysis (extracellular
              rates = ECAR) in living cells, enabling systematic characterization of their metabolic f

              Samples are analyzed in a 96-well microtiter plate format and measurements are performed
              optical principle with LEDs and optical sensors.