Vevo 3100 ****************************************************************************************** * Ultrasound Imaging System ****************************************************************************************** Supplier accela s.r.o. Year of acquisition 2018 Price 14.6 million. CZK Financing OP VVV CORE FACILITIES [ URL "LFHKEN-192.html "] CZ.02.1.01/0.0/0.0/16_017/0002515 Responsible Person doc. PharmDr. Martin Štěrba, Ph.D. In vivo experiments on experimental animals are still a key and indispensable part of medi because this approach, unlike in vitro experiments, reflects the complex environment of th organism. Non-invasive imaging methods are an integral part of modern experimental researc as they can provide key anatomical and functional data. Non-invasive ultrasound (US) imaging has had a firm place in clinical medicine for many de approach has extraordinary translational potential because the same or similar parameters practice can be evaluated in an experimental model. Other advantages of ultrasound include performing repeated examinations with minimal burden on the experimental animal and neglig costs. With ultrasound, the development of a pathological condition and its therapeutic in by a drug or surgical intervention) can be monitored in an experiment. Compared to invasiv approaches, this approach is therefore considerably more gentle on the experimental animal significantly reduce the number of experimental animals, which is in line with current tre requirements ('reduction' and 'refinement'). Most in vivo biomedical research today is carried out on small laboratory animals -usually (especially mice and rats) and rabbits. However, the use of small laboratory animals place technical demands on the instrumentation. For example, the imaging capabilities for echoca examination of mice must be prepared and optimized to ensure that the longitudinal dimensi mouse heart is less than 1 cm and the transverse dimension is less than 0.5 cm. The physio rate of the mouse is 450-650 beats/min. In addition, the examination must be performed und anaesthesia with careful monitoring of vital signs. In response to these specific requirements of experimental research, special high-frequenc equipment was developed for experiments on small laboratory animals only. An example is the state-of-the-art VEVO 3100 high-frequency ultrasound set-up, complete wi of probes (18-70 MHz, with resolution up to 30 ?m) for use on laboratory rodents (mouse, r and rabbit. The assembly forms a functional unit that allows a wide application of the ins experimental in vivo research. The assembly is designed for use by multiple research group Facility" format. *========================================================================================= * The instrumentation consists of: *========================================================================================= 1.High-frequency UZ VEVO 3100 with LCD screen and touchscreen with relevant software and s packages for individual applications + VevoLab software for off-line evaluation. Hardwar are fully prepared for basic and more advanced ultrasound examinations (including 3D, ul contrast, speckle tracking). 2.Full range of ultrasound probes: 1. MX201 18 MHz (10-21 MHz, max. resolution 100 ?m, max max. 367 FPS), 2. MX250 24 MHz (14-28 MHz, max. resolution 75 ?m, max. depth 3 cm, max. 3. MX250 24 MHz (14-28 MHz, max. 367 FPS), 3. MX400 38 MHz (21-44 MHz, max. resolution 5 2 cm, max. 449 FPS), 4. MX550D 50 MHz (26-52 MHz, max. resolution 40 ?m, max. depth 1.5 FPS), and 5. MX700 70 MHz (30-70 MHz, max. resolution 30 ?m, max. depth 1.2 cm, max. 476 3.Vevo Imaging Station 2 – apparatus for fixing the animal and probe and moving both the a probe in all axes (for most examinations this is a necessity - examination directly "by demanding and not very reproducible). Module for ultrasound guided injection. 4.Heated plate for mouse and rat with vital signs monitor (rectal temperature, ECG, heart rate are monitored on-line, displayed on the LCD monitor of the ultrasound machine and s with the recording). 5.Inhalation anaesthetic unit for isoflurane anaesthesia with oxygen for breathing, includ chamber and exhaust to absorber. Examples of experimental research areas where the instrument can be used: • Cardiovascular research – basic and advanced examination of the structure and function o (including e.g. myocardial strain examination) and blood vessels - applications in resea infarction, myocardial hypertrophy, heart failure, cardiotoxicity, cardiomyopathy, valvu atherogenesis. • Tumors – orthotopic and s.c. implanted tumor imaging, evaluation of growth /3D volume, a tumor perfusion, controlled injection into the tumor. • Embryo-fetal applications – early detection/confirmation of pregnancy, teratogenicity st and xenobiotics, fetal programming, ultrasound-guided puncture of amniotic fluid and lef intra-amniotic inflammation, placental structure and function, including assessment of m fetal perfusion. • Liver disease – alcoholic and non-alcoholic fatty liver disease, hepatotoxicity, hepatop regeneration, changes in liver perfusion and liver metastasis. • Kidney disease – research on renal dysfunction, renal perfusion, chronic kidney disease, kidney disease, hypertension, hydronephrosis and renal cell carcinoma. Galleries from the examination with this device are located on the manufacturer's website: www.visualsonics.com/product/imaging-systems/vevo-3100 [ URL "https://www.visualsonics.com systems/vevo-3100"] . The manufacturer provides advanced hands-on training and instruction for individual applic Author:: doc. PharmDr. Martin Štěrba, Ph.D.