IVIS Spectrum ****************************************************************************************** * In Vivo Imaging System ****************************************************************************************** Supplier PE Systems s.r.o. Year of acquisition 2018 Price 12.7 milion. CZK Financing OP VVV CORE FACILITIES [ URL "LFHKEN-192.html "] CZ.02.1.01/0.0/0.0/16_017/0002515 Responsible Person prof. MUDr. Stanislav Mičuda, Ph.D. Developing new drugs, discovering and synthesising new compounds that could help us fight goal of many research teams around the world. This process is lengthy, challenging and can During this process, it is important to find out whether a newly discovered substance has effect, whether it kills cancer cells or merely slows down their growth, etc. In the early development, we test candidate substances in vitro, where we observe these processes on in and try to unravel the mechanism of action at the molecular level. If at this stage the su to have promising properties, it can then be taken to the next step, which is to see how t whole reacts to the substance. In vivo testing in model organisms serves this purpose. The priority for us is not only to find out how the substance will affect a healthy organi a quantity might be harmful, but more importantly how effective it will be specifically ag growth. Since in model organisms (mice, rats,...) the occurrence of tumours is random and need to reach a state where we can test the substance reliably, i.e. to create "tumour mod see the effect of the substance on the tumour cells we choose. Comment: Why do I need to use this procedure? There are many processes and processes in a living or influence and enable the organism to survive and adapt, whether to the environment or to e These processes may also influence the fate of the test substance in the organism. They ma absorption, metabolism, mechanisms of action or excretion. *========================================================================================= * Imaging *========================================================================================= Now we come to the question, how to non-invasively monitor, the effectiveness of the subst has the tumour stopped growing or has it shrunk, for example? In the past (and sometimes even today), the size of subcutaneous tumours was (is) measured callipers, which is an instrument that can be used to determine the dimensions of the tumo calculate its volume. Modern medicine, however, has more modern means, based primarily on techniques. These allow us to look inside the body without violating its integrity or inte invasive way. Classical imaging techniques may include CT, MRI, X-ray or ultrasound, adapt small laboratory animals. A more advanced option is to observe targeted structures within the organism. Some devices use the principle of luminescence or fluorescence to image spec cells. By binding specific fluorescent or luminescent markers in the target structure of t cells and then binding these markers, these cells and structures can be imaged. This is ma for example, by the unique IVIS Spectrum In Vivo Imaging System from Perkin Elmer. The ins both fluorescence (the emission of radiation induced by another excitation radiation and p nanoseconds after the excitation has ended) and bioluminescence (the ability of organisms e.g. enzymatically by luciferase to cleave D-luciferin to produce yellow-green light). Key features and benefits of the IVIS Spectrum imager • High sensitivity in vivo fluorescence and bioluminescence imaging • Multi-object analysis (5 mice) with 23 cm field of view • High resolution (up to 20 microns) with a 3.9 cm field of view • Twenty-eight high-efficiency filters with a range of 430 - 850 nm • Ideal for resolution of multiple bioluminescent and fluorescent reporters (labeling) • 3D diffusion tomographic reconstruction for both fluorescence and bioluminescence • Ability to import and automatically copy CT or MRI images with functional and anatomical • Inhalation anaesthesia inlet and outlet ports One of the interesting and useful functions of the instrument is the creation of a 3D spat reconstruction of the observed object. This function works on the principle of creating a images, from which the instrument then composes a three-dimensional model, on which the ex the observed object (for example, a tumor) can be tracked, with possible measurement of si and dimensions. The locations of the individual organs from the available libraries can th the three-dimensional models to complete the integrity of the observed model. There are many possible applications of the instrument. It is possible to observe individu cells in a plate/tube or whole live models, especially of laboratory mice of different spe a luminescent or fluorescent label to a specific antibody that can be directed to a variet in the imaged model, we are then presented with a much wider range of possibilities in the structures and tissues, whether healthy or pathologically altered, in a living organism. I should also be possible to observe certain ongoing infections. The instrument has great po possibilities of its use will certainly grow in the future. At present, our department is the Czech Republic that has such a device. Author of the text: RNDr. Martin Uher Source: IVIS Spectrum In Vivo Imaging System. PerkinElmer [online]. Waltham: PerkinElmer, 2019 [ci