Agilent Seahorse












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            * XFe96 Analyzer Power Pak
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            Supplier               HPST, s.r.o.                             
            Year of acquisition    2018                                     
            Price                  7.6 milion. CZK                          
                                                                            
            Financing              OP VVV CORE FACILITIES [ URL "LFHKEN-192.html "]
                                   CZ.02.1.01/0.0/0.0/16_017/0002515        
            Responsible Person     doc. MUDr. Otto Kučera, Ph.D.            


            At the department of experimental hepatology, we have long focused on toxic liver injury, 
            mechanisms of action of model hepatotoxins and substances with potential hepatoprotective 

            hepatic regeneration induced by partial hepatectomy or toxic liver injury, sensitivity of 
            hepatotoxic agents and regenerative response in the field of liver affected by non-alcohol
            disease (NAFLD, NASH), extrahepatic cholestasis induced by ligation d. choledochus, and th

            influencing the development of subsequent liver injury.

            The liver is an important organ of the body, it performs, among other things, a number of 

            functions in intermediary and energy metabolism, it is the main site of biotransformation 
            of xenobiotics and many substances of endogenous origin. The importance of proper assessme
            mitochondrial function is emphasized by findings that document that impaired hepatic mitoc

            accompanies most common metabolic, nutritional, and other diseases of civilization. Mitoch
            can be studied in a variety of models including liver homogenate, mitochondria and hepatoc
            from the liver under in vivo conditions; isolated hepatocytes, stabilized cell lines or tu

            can be used in in vitro experiments. For the assessment of mitochondrial function, it is e
            instrumentation and standard operating procedures harmonised with other laboratories to al
            results obtained at other sites.


            In our laboratory, we have so far used high-performance respirometry using the Oroboros-2k
            appropriately combining different substrates, extenders and specific inhibitors, the oxygr

            assessment of different biotransformation energy pathways, individual respiratory complexe
            (respiratory control index, ATP production rate, integrity of the outer and inner mitochon
            Tissue homogenates, intact or permeabilized cells, isolated mitochondria and permeabilized

            analyzed. The disadvantage is that this instrument allows simultaneous analysis of only 2 
            oxygraph and it is not possible to analyze cells adhered to the surface.


            The Core Facilities project allowed us to acquire a unique instrument for cellular metabol
            Agilent Seahorse Bioscience XFe96 analyzer.