Department of Medical Biology and Genetics












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            Department of Medical Biology and Genetics provides courses of basic subject Medical Biolo

            programs and in both Czech and English languages. In addition to teaching and educational 
            main tasks of the Department include scientific research activities. These focus on basic 
            research in the field of cell, molecular biology and genetics, traditionally using in vitr

            (cell cultures 2D and 3D formats) since the Department is one of the pioneers of these met
            Czech Republic. In addition, recently, the mentioned methods have been expanded to include
            (nude mouse) too. The main focus of research projects is based on traditionally studied to

            mechanisms of stress responses of cellular systems to selected xenobiotics as well as micr
            selenium). The main current area of interest is the research of selected phenotypic proper

            cells (melanoma, glioblastoma, pancreatic cancer) related to the aggressive nature of thes
            spread and resistance. The research uses a full spectrum of methodological approaches incl
            advanced morphometric and cytometric approaches, including time-lapse studies performed on

            which are unique within the entire medical faculty in Hradec Kralove.





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            * Main research topics
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            • Mechanisms of stress response to exposure to selected xenobiotics and micronutrients (st

              signaling pathways and spectrum of cellular responses - death, senescence, autophagy).
              Prof. PharmDr. Emil Rudolf, Ph.D [ URL "https://is.cuni.cz/webapps/whois2/osoba/10755514
              lang=en"]

            • Study of STAT3 signaling as a mechanism of resistance as well as the target of intervent
              cells (glioblastoma, pancreatic cancer).).
              RNDr. Veronika Skarková, Ph.D. [ URL "https://is.cuni.cz/webapps/whois2/osoba/1319719446

              lang=en"] , Prof. PharmDr. Emil Rudolf, Ph.D.







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            * Equipment
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            • morphological and cytometric laboratory - motorized fluorescence microscope Nikon Eclips
              ImageXpress Micro XLS cytometer enabling analysis and long-term monitoring of morphologi
              molecular changes in the studied cell system

            • cell culture laboratory enabling the derivation and cultivation of cell systems
              in vitro/ex vivo, in 2D and in 3D models, including a wide range of cellular and biochem
              (spectrofluorimeter TECAN, XCelligence RTCA DP)

            • functional laboratory for genetic and molecular analysis via DNA, RNA or protein express
              intervention(PCR including quantitative - Light cycler Roche 96, Nanodrop, Azure C600 de







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            * Scientific research groups

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            All academic staff of the department, including undergraduate and postgraduate students, a
            the specified scientific research topics. At the same time, they also participate in and c

            projects and scientific tasks solved by other departments within the Faculty of Medicine i
            Hradec Králové University Hospital and other extramural domestic and foreign institutions.