Experimental Hepatology Laboratory ****************************************************************************************** * ****************************************************************************************** At the Experimental Hepatology group, we focus on toxic liver damage, hepatoprotection, li and, more recently, Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD). The liver is the main metabolic organ that coordinates whole-body homeostasis and serves a site for the biotransformation of xenobiotics and various endogenous substances. Liver mit a crucial role in these processes, and their dysfunction accompanies most liver pathologie considered the hepatic manifestation of metabolic syndrome and is currently the most commo disease. Some authors even consider MAFLD a mitochondrial disease. Studying mitochondrial the possibility of its modulation is, therefore, essential for understanding pathological exploring diagnostic and therapeutic options for liver diseases. Our department uses various model systems to study liver function, including liver homogen mitochondria, isolated hepatocytes, stabilized, and cancer cell lines—each with its advant limitations. Recently, evidence has emerged suggesting that the energy metabolism of plate the overall metabolic state of the organism/body, including liver function. Since platelet tissue, are easily accessible biological material, assessing their energy state could be i diagnosing liver damage and monitoring the effects of potential therapies. To evaluate mit function, it is essential to have the necessary equipment and standardized protocols harmo laboratories. Our laboratory has been equipped with three Oroboros-2k oxygraphs. By using a suitable com different substrates, uncouplers, and specific inhibitors, the oxygraph enables the assess energy pathways, individual respiratory complexes, and functions (such as the respiratory ATP production intensity, and the integrity of the outer and inner mitochondrial membranes the oxygraph with fluorescent sensors, we can also evaluate mitochondrial membrane potenti oxygen species production, and calcium retention capacity. However, a limitation of this d allows simultaneous analysis of only two samples per oxygraph and does not support the ana cells. Thanks to the Core Facilities [ URL "https://www.lfhk.cuni.cz/LFHK-192.html"] project, we expanded our unique equipment with an Agilent Seahorse Bioscience XFe96 analyzer for cellu analysis. Its advantage lies in the ability to simultaneously analyze adherent samples in while assessing both mitochondrial respiration and glycolysis intensity. However, the limi small number of substrate additions, which limits more detailed analysis. Therefore, the i is to combine both methods.Recently, through the Ph.D. Infra for CU [ URL "https://www.lfh LFHK-188.html"] project, we have also acquired the NextGen-O2k device, which is compatible oxygraphs. This upgrade will enable us to analyze eight samples simultaneously, which is c the time-intensive nature of these analyses. The device is also equipped with a Q-module, simultaneous assessment of the redox state of coenzyme Q using cyclic voltammetry. Coenzym role in the respiratory chain, and changes in its redox state have been linked to various All our instruments are also available to other departments for the analysis of energy par various cell types and tissues. ****************************************************************************************** * Agilent Seahorse Bioscience XFe96 Analyzer Power Pak ****************************************************************************************** Supplier HPST, s.r.o. Acquisition year 2018 Price 7.6 million CZK incl. VAT Funding P RDE CORE FACILITIES [ URL "https:// www.lfhk.cuni.cz/LFHK-192.html"] CZ.02.1.01/0.0/0.0/16_017/0002515 Person responsible doc. MUDr. Otto Kučera, Ph.D. [ URL "https:// is.cuni.cz/webapps/whois2/osoba/1967794929583787/? lang=en"] ****************************************************************************************** * Oroboros NextGen-O2k Startup, Series XB vč. O2k-Q-Module ****************************************************************************************** Supplier Oroboros Instruments GmbH Acquisition year 2025 Price 2.9 million CZK incl. VAT Funding P JAC Ph.D. Infra for CU [ URL "https:// www.lfhk.cuni.cz/LFHK-188.html"] CZ.02.01.01/00/22_012/0005514 Person responsible Mgr. Pavla Staňková, Ph.D. [ URL "https://is.cuni.cz/ webapps/whois2/osoba/1442971236463993/?lang=en"]