Department of Medical Biochemistry ****************************************************************************************** * Main research topics ****************************************************************************************** • Research on candidate anticancer drugs and experimental oncology • Development of new mass spectrometric methods for pharmacokinetic, toxicokinetic and met • Screening for the antiproliferative effect of newly synthesized, isolated and modified c subsequent study of the mechanism of antitumor effect in selected promising compounds • Study of the mechanism of resistance to standard chemotherapy in selected tumour lines • Metabolomic analysis of bile acids • Biocompatibility testing of novel composite nanomaterials ****************************************************************************************** * Equipment ****************************************************************************************** Cell laboratories for the cultivation of tumor lines, equipment for the determination of c protein expression (BioRad), a system for real-time monitoring of cell proliferation (Xcel high-throughput imaging system for monitoring various cellular functions (ImageXpress), a plate reader (Tecan Spark), a flow cytometer with four excitation lasers and fourteen fluo detectors (Beckman Coulter), a qPCR cycler for quantification of nucleic acids in tissues (Quantstudio 6). Acquity I-Class liquid chromatograph coupled to a Xevo TQ-XS triple quadrupole mass detect analysis of drugs, metabolites and endogenous compounds in biological matrices. The focus determination of drug levels in pharmacokinetic or toxicokinetic studies, for therapeutic and in bile acid screening for basic and applied research mainly in the field of liver pat development. ****************************************************************************************** * Scientific research groups ****************************************************************************************** *========================================================================================= * Research on candidate anticancer drugs and experimental oncology *========================================================================================= Head: prof. MUDr. Martina Řezáčová, PhD.  [ URL "https://is.cuni.cz/webapps/whois2/osoba/1 lang=en"] Key Personnel: doc. RNDr. Radim Havelek, PhD. [ URL "https://is.cuni.cz/webapps/whois2/ osoba/1600205573620347/?lang=en"] , PharmDr. Darina Muthná, PhD. [ URL "https://is.cuni.cz osoba/1504977622007434/?lang=en"] , Mgr. Alena Mrkvicová, PhD. [ URL "https://is.cuni.cz/w osoba/1126518199213415/?lang=en"]   The main research topic of the group is experimental oncology, in which we focus on severa The first one is the study of novel candidate structures for cancer pharmacotherapy. This screening for antiproliferative effects of compounds of different origin. Among the synthe these include low molecular weight kinase inhibitors and metallocomplexes, and among subst origin, plant alkaloids and their synthetic or semi-synthetic analogues. Selected promising compounds are further tested in more detail to elucidate the possible m anticancer action. A second key area of research is the study of mechanisms leading to res cells to standard chemotherapy. And the last research topic is to investigate the cytotoxi magnetic nanosystems and their potential use for drug transport. All research topics use m testing methods that rely on in vitro experiments with selected tumor lines. *========================================================================================= * Mass spectrometric analysis laboratory: PharmaMassSpec *========================================================================================= Head: doc. Ing. Miloš Hroch, Ph.D.  [ URL "https://is.cuni.cz/webapps/whois2/osoba/1192254 lang=en"]   The laboratory specializes in the development, optimization and validation of analytical m quantitative and qualitative analysis of substances from groups of drugs, metabolites and substances. Our long-term focus is on bile acid analysis and analysis of anticancer drugs. Another branch of research is focused on the implementation of 3D printing and the use of statistical methods in the field of analytical chemistry. All these methodologies are appl research of new drugs, the search for new indications for established drugs, therapeutic m levels in cancer research and other areas. *========================================================================================= * Cooperations *========================================================================================= LFHK UK: prof. MUDr. Stanislav Mičuda, Ph.D. [ URL "https://is.cuni.cz/webapps/whois2/?SEA %C4%8Duda&lang=en"]   FaF UK: PharmDr Petr Pávek, Ph.D., prof. PharmDr. Petr Nachtigal, Ph.D. FN HK: PharmDr. Martin Mžik, Ph.D., MUDr. Martin Šimkovič, Ph.D., MUDr. Dominika Écsiová University Hospital Hradec Králové, Biomedical Research Centre Institute of Physics of the CAS, Department of Magnetics and Superconductors Charles University in Prague, Faculty of Pharmacy, Department of Pharmacognosy and Pharmac Charles University in Prague, Faculty of Pharmacy, Department of Pharmacology and Toxicolo Palacký University in Olomouc, Faculty of Science, Department of Inorganic Chemistry University of Pardubice, Faculty of Chemical Technology, Department of Biological and Bioc University of Pardubice, Faculty of Chemical Technology, Department of General and Inorgan University of Defence, Faculty of Military Medicine, Department of Radiobiology